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Preparation of Digoxigenin labeled riboprobes
Preparation of riboprobes useful for in situ hybridization
DNA extraction from whole blood
DNA extraction from whole blood using a salting out procedure
Inverse-PCR (I-PCR) for the identification of the breakpoints of deletions
The Inverse-PCR is a method that allows to amplify unknown regions of DNA, starting from the flanking known region on which are positioned the primers, in inverse direction compared to a normal PCR. It is necessary circularizing the DNA fragments, using ligase, then perform the I-PCR.
Circularization of DNA fragments for the molecular characterization of new deletions
During the molecular characterization of new deletions (to define the breakpoints) can occur that one extreme is known, but not the second one. In these cases it is very useful and suitable to perform the circulatization by ligase of DNA fragments containing the beakpoints of the deletion, followed by the inverse-PCR. Using these methods we were able to define the breakpoints of two new beta-Thalassemia deletions.
RNA riboprobe synthesis
T7 RNA polymerase transcription
SELEX methodology for cell-specific aptamers for targeted therapies
Selex protocol for selection of GL21.T and Gint4.T and CL4 aptamers
Cytoplasmic Protein extraction
S100 cytoplasmic extract
Preparation of radioactive probes for nucleic acids blots
It generates highly specific and hot probes.
Magnetic labeling and isolation of biotinylated molecules
A fishing/competition approach used to validate the interaction between lncRNAs and microRNAs by using biotinylated Peptide Nucleic Acid (PNA) oligomers as probes.
