Transcription mix 600ul
| Reagents | F-concentration | Quantity | I-concentration |
|---|---|---|---|
|
T7 Transcription buffer
|
1X
|
120 uL
|
5X
|
|
2'F-2'-dCTP
|
1mM
|
60 uL
|
10mM
|
|
2'F-2'-dUTP
|
1mM
|
60 uL
|
10mM
|
|
ATP
|
1mM
|
60 uL
|
10mM
|
|
GTP
|
1mM
|
60 uL
|
10mM
|
|
DTT
|
16,7 mM
|
100 uL
|
100mM
|
|
RNase inhibitors
|
28 U/uL
|
15 uL
|
40 U/uL
|
|
T7 RNA Pol.
|
1,5 U/uL
|
6 uL
|
50 U/uL
|
|
Inorganic Pyrophosphatase
|
1 U/uL
|
3 uL
|
200U/uL
|
|
Template
|
0,5 pmol/uL
|
||
|
Water
|
to 600 uL
|
