Hypotonic Buffer
Reagents | F-concentration | Quantity | I-concentration |
---|---|---|---|
HEPES, pH 7.9
|
10 mM
|
||
MgCl2
|
1.5 mM
|
||
KCl
|
10mM
|
||
PMSF
|
0,2 mM
|
||
PI (protease inhibitors cocktail ROCHE)
|
1x
|