Inverse-PCR mix
| Reagents | F-concentration | Quantity | I-concentration |
|---|---|---|---|
|
Buffer
|
1X
|
2.5 μl
|
10X
|
|
Primer For
|
0.4 μM
|
10-12 pmol
|
|
|
Primer Rev
|
0.4 μM
|
10-12 pmol
|
|
|
dNTP
|
0.2 mM
|
4 μl
|
1.25 mM
|
|
Taq Polimerasi
|
0.125 μl
|
5 U/μl
|
|
|
H2O bd
|
up to 25 μl
|
The primers used are in inverse direction
