Restriction Enzyme reaction mix (40μl)
| Reagents | F-concentration | Quantity | I-concentration |
|---|---|---|---|
|
DNA
|
15 μg
|
||
|
Restriction enzyme
|
1 μl
|
20U/μl
|
|
|
Buffer 10X
|
4 μl
|
10X
|
|
|
H2O
|
up to 40 μl
|
