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Home  |  Protocols  |  Molecular Biology  |  SELEX methodology for cell-specific aptamers for targeted therapies  |  Loading solution 500ul

Loading solution 500ul


Reagents F-concentration Quantity I-concentration
Deionized Formamide
Formamide 95%
475 ul
Formamide 99,5%
RNasi free water
10 ul
EDTA pH 8.0
EDTA 0,01M pH 8.0
10 ul
EDTA 0,5M pH 8.0
Bromophenol

N.B Formamide oxidies readily in air and should be deionized by passage through a mixes-bed resin (Bio-Rad AG 501-X8) until its pH is neutral. It is then recrystallized at 0°C and stored at -20°C in small aliquots in tightly capped tubes

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