Loading solution 500ul
Reagents | F-concentration | Quantity | I-concentration |
---|---|---|---|
Deionized Formamide
|
Formamide 95%
|
475 ul
|
Formamide 99,5%
|
RNasi free water
|
10 ul
|
||
EDTA pH 8.0
|
EDTA 0,01M pH 8.0
|
10 ul
|
EDTA 0,5M pH 8.0
|
Bromophenol
|
N.B Formamide oxidies readily in air and should be deionized by passage through a mixes-bed resin (Bio-Rad AG 501-X8) until its pH is neutral. It is then recrystallized at 0°C and stored at -20°C in small aliquots in tightly capped tubes