10x cytoplasmic extraction buffer
Reagents | F-concentration | Quantity | I-concentration |
---|---|---|---|
HEPES, pH 7.9
|
0,3M
|
||
KCl
|
1,4 M
|
||
MgCl2
|
0,03M
|
||
PI (protease inhibitors cocktail ROCHE)
|
1X
|
Add PI before use