1x binding buffer
| Reagents | F-concentration | Quantity | I-concentration |
|---|---|---|---|
|
HEPES pH 7.6
|
10 mM
|
||
|
MgCl2
|
3 mM
|
||
|
glycerol
|
5%
|
||
|
KCl
|
40 mM
|
||
|
DTT
|
1 mM
|
||
|
protease inhibitors
|
1x
|
