Clone Chart - pGEM LAC Z 1
It is used to obtain a LacZ riboprobe
Category:
Plasmid
Arrival or creation date: Jan 13, 2006
Last revision: Dec 18, 2014
Author(s):
F. Anna Digilio, Daniela Cavaliere
Contact
Name: F. Anna Digilio
Address: Institute of Biosciences and BioResources - CNR, Via P. Castellino 111, 80131 Naples, Italy
Phone: +39 081 6132430
Email: anna.digilio@ibbr.cnr.it
Vector: pGem T easy vector
Resistances: Ampicillin
Bacterial Strain: DH5a
Insert: E. coli beta galactosidase (lacZ) gene; 670 bp fragment amplified by using two internal primers
Description of cloning procedure:
Amplified by PCR with LacZ F and LacZ R primers on the E.coli LacZ gene and cloned in pGEM T easy vector
Intended Use: It has been used to obtain both sense and anti-sense DIG-labeled "LacZ riboprobes"
Special Remarks: To obtain the antisense riboprobe, you can linearize with the Sal1 restriction enzyme and retro-transcribe by using T7 RNA polymerase; for the sense riboprobe, useful as negative control, digest with the NcoI RE and retrotranscribe with the SP6 RNA pol.
Quality validation:
Yes
Validation info
The construct has been used to obtain data published in a peer-reviewed journal
Funded by: Grants from the Regione Campania (Law n. 5)
Related Protocols:
Related Oligos:
