Clone Chart - pCR-Cry-3
mouse Cryptic riboprobe for in situ hybridization
Category:
Plasmid
Last revision: Dec 16, 2014
Author(s):
Giovanna L. Liguori
Contact
Name: Giovanna L. Liguori
Address: Institute of Genetics and Biophysics "A. Buzzati-Traverso", CNR. Via Pietro Castellino 111, 80131 Napoli
Email: giovanna.liguori@igb.cnr.it
Vector: pCR2.1.TOPO, 3.9 kb
Resistances: Amp, Kan
Insert: mouse Cryptic cDNA from 250 to 683 bp (NCBI sequence NM_007685.2), 472 bp long, amplified by PCR on F9 teratocarcinoma cell line cDNA
Description of cloning procedure:
Direct cloning of the PCR product
Intended Use: In Situ Hybridization
Special Remarks: For antisense probe cut BamHI and transcribe with T7 RNA polymerase
Quality validation:
Yes
Validation info
The plasmid has been checked by restriction analysis and sequence and then used to syntethize Cryptic probe for whole mount in situ hybridization experiments on mouse embryos. The data obtained have been published in peer-reviewed journals.
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