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Home  |  Molecular Tools  |  Oligos  |  LacZ F
Home  |  Molecular Tools  |  Oligos  |  LacZ F

Oligo - LacZ F

Useful to amplify, in pair with lacZ R, a fragment from the E. coli lacZ gene

CategoryDNA
Creation dateJan 13, 2005
Last revisionDec 17, 2014
Author(s)F. Anna Digilio, Rosa Morra
Contact
NameF. Anna Digilio
AddressInstitute of Biosciences and BioResources - CNR, Via P. Castellino 111, 80131 Naples, Italy
Phone+39 081 6132430
Emailanna.digilio@ibbr.cnr.it

Sequence

TCATCTGTGGTGCAACGG

 

Orientationforward
Position NCBI Reference Sequence: NC_000913.3:365853 -365836; +453 in the lacZ gene from Escherichia coli strain K12
Length (nt)18
Melting Temperature (°C)56°C
% GC55,6%
Degenerationno
Intended UsePCR AMPLIFICATION

 

Quality validation: Yes

Validation info

The oligo has been used to obtain data published in peer-reviewed journals.

 

Citations
Di Cara F, Cavaliere D, Galliero V, Polito LC, Digilio FA. Expressional and functional analysis of the male-specific cluster mst36F during Drosophila spermatogenesis. Insect Mol Biol.2010;19:807-813
Di Cara F, Morra R, Cavaliere D, Sorrentino A, De Simone A, Polito CL, Digilio AF.Structure and expression of a novel gene family showing male germline specific expression in Drosophila melanogaster.Insect Mol Biol. 2006;15:813-822

 

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