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Home  |  Protocols  |  Molecular Biology  |  SELEX methodology for cell-specific aptamers for targeted therapies  |  PCR mix 300ul

PCR mix 300ul


Reagents F-concentration Quantity I-concentration
Buffer PCR
1X
30 uL
10X
dNTP
0,2 mM
6 uL
10mM
Rev Primer
0,2 uM
0,6 uL
100 uM
Fw Primer
0,2 uM
0,6 uL
100 uM
TAQ
0,05 U/uL
5 uL
5 U/uL
Template
60 ng
Water
to 300 uL

N.B The quantity of MgCL2 is variable

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