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Home  |  Model Systems  |  Vertebrates  |  Mouse  |  Cripto (Tdgf1) null mutants

Model System - Cripto (Tdgf1) null mutants

The Cripto +/- mice are healthy and fertile. The Cripto -/- mutants dye during embryo development at about 9.0 dpc, due to gastrulation failure

CategoryVertebrate  /  Mouse
Arrival or creation dateJan 07, 1999
Last revisionDec 18, 2014
Author(s)Chunhui Xu, Giovanna L. Liguori, M. Graziella Persico and Eileen D. Adamson
Contact
NameGiovanna L. Liguori
AddressInstitute of Genetics and Biophysics A. Buzzati-Traverso - CNR, Via P. Castellino 111, 80131 Naples, Italy
Phone0816132294
Emailgiovanna.liguori@igb.cnr.it

 

Figure legend:

Double WISH experiments on mouse embryos at 8.5 dpc showing that Cripto null mutants express anterior neural markers, but oriented along a Proximal-Distal axis instead of an Anterior-Posterior one.


GenotypeCripto +/-
OriginThe Burnham Institute, La Jolla Cancer Research Center, 10901, N. Torrey Pines Road, La Jolla, CA 92037, USA

Description

The mutants have been created by homologous recombination in ES cells and following blastocist injection. The gene targeting has been obtained by replacement of the Cripto gene exons 3, 4 and 5 by the PGK-neo cassette  (Xu et al., 1999).
The Cripto -/- mutants dye during embryo development at about 9.0 dpc, due to gastrulation failure and almost complete absence of mesendoderm derivatives (Ding et al., 1998; Xu et al., 1999; Liguori et al., 2003). Interestingly, the embryos are mostly constituted by anterior neuroectderm, which still retain its molecular and functional characteristics (Liguori et al., 2003; 2009).

The phenotype has been studied both in a C56Bl6 background and in a mixed background constituted by C56Bl6, Balck Swiss and Sv129J.

The Cripto +/- mice are healthy and fertile, but are less susceptible than wt to carcinogen treatment (Giorgio et al., 2014).

 

Quality validation: Yes

Validation info

The generation, characterization and phenotypic analysis of Cripto mutants have been published in peer-reviewed journals (see citations).

 

Citations
Giorgio E, Liguoro A, D'Orsi L, Mancinelli S, Barbieri A, Palma G, Arra C, Liguori GL. Cripto haploinsufficiency affects in vivo colon tumor development. Int J Oncol. 2014;45:31-40.
Liguori G.L., Echevarria D., Bonilla S., D’Andrea D., Liguoro A., Persico M.G., Martinez S. Characterization of the functional properties of the neuroectoderm in mouse Cripto-/- embryos showing severe gastrulation defects. Int J Dev Biol. 2009; 53: 549-57
Liguori G.L., Echevarria, D., Improta, R., Signore, M., Adamson, E., Martinez, S., Persico, M.G. Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak. Dev Biol. 2003; 264: 537-49.
Xu, C., Liguori, G., Persico, M.G. and Adamson, E.D. Abrogation of the Cripto gene in mouse leads to failure of postgastrulation morphogenesis and lack of differentiation of cardiomyocytes. Development. 1999; 126:483-94.

 

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