Model System - Cripto (Tdgf1) null mutants
The Cripto +/- mice are healthy and fertile. The Cripto -/- mutants dye during embryo development at about 9.0 dpc, due to gastrulation failure
Category:
Vertebrate
/
Mouse
Arrival or creation date: Jan 07, 1999
Last revision: Dec 18, 2014
Author(s):
Chunhui Xu, Giovanna L. Liguori, M. Graziella Persico and Eileen D. Adamson
Contact
Name: Giovanna L. Liguori
Address: Institute of Genetics and Biophysics A. Buzzati-Traverso - CNR, Via P. Castellino 111, 80131 Naples, Italy
Phone: 0816132294
Email: giovanna.liguori@igb.cnr.it
Figure legend:
Double WISH experiments on mouse embryos at 8.5 dpc showing that Cripto null mutants express anterior neural markers, but oriented along a Proximal-Distal axis instead of an Anterior-Posterior one.
Genotype: Cripto +/-
Origin: The Burnham Institute, La Jolla Cancer Research Center, 10901, N. Torrey Pines Road, La Jolla, CA 92037, USA
Description
The mutants have been created by homologous recombination in ES cells and following blastocist injection. The gene targeting has been obtained by replacement of the Cripto gene exons 3, 4 and 5 by the PGK-neo cassette (Xu et al., 1999).
The Cripto -/- mutants dye during embryo development at about 9.0 dpc, due to gastrulation failure and almost complete absence of mesendoderm derivatives (Ding et al., 1998; Xu et al., 1999; Liguori et al., 2003). Interestingly, the embryos are mostly constituted by anterior neuroectderm, which still retain its molecular and functional characteristics (Liguori et al., 2003; 2009).
The phenotype has been studied both in a C56Bl6 background and in a mixed background constituted by C56Bl6, Balck Swiss and Sv129J.
The Cripto +/- mice are healthy and fertile, but are less susceptible than wt to carcinogen treatment (Giorgio et al., 2014).
Quality validation:
Yes
Validation info
The generation, characterization and phenotypic analysis of Cripto mutants have been published in peer-reviewed journals (see citations).
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