Model System - LAN-5 human, neuroblastoma
Continuous culture, grown as monolayer, neuroblast-like morphology
Category:
Cells
/
Cell lines
Arrival or creation date: Dec 20, 2013
Last revision: Jan 29, 2015
Author(s):
Contact
Name: Marta Di Carlo
Address: IBIM - CNR via Ugo La Malfa, 153, Palermo
Phone: 0916809538
Email: di-carlo@ibim.cnr.it
Origin: human neuroblastoma
Description
How to mantain:
The LAN-5 nueroblastoma cell line are maintained in RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids, 1% antibiotic (penicillin and streptomycin). Cells are mantained at 37°C in a humidified atmospere with 5% CO2
Freezing medium: 90% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2; after thawing the cells look very suffering, tend to form clusters and need about one week to attach; thaw with medium supplemented with 20% FBS; 37C, 5% CO2.
The cells grow very slowly
Quality validation:
Yes
Validation info
Publication in peer-reviewed journals
