Model System - FRT
Fischer Rat Thyroid epithelial cell line
Figure legend:
Microphotograph of FRT cells in standard growing condition. Tridimensional structures formation called dome in confluent cells (white arrows).
Description
FRT are poorly differentiated epithelial cell line derived from Fischer rat thyroid gland.
FRT cells are well polarized both morphologically and functionally and among the thyroid markers expresse only Pax8 a paired domain containing protein found in thyroid cells, kidney and brain.
FRT cell line represent a powerful tool to investigate the molecular mechanisms responsible for the acquisition and maintenance of polarity in epithelial cells, the protein trafficking/sorting in polarized cells and the control thyroid specific gene transcription.
Culture conditions:
FRT cells were cultured in Coon’s modified Ham’s F-12 medium supplemented with 5% fetal calf serum. Cultured cells were maintained in a humidified atmosphere of 5% CO2 and 95% air, at 37°C.
Validation info
The model system has been characterized and published in peer-reviewed journals.
Funded by:
