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Model System - EPN

Human non-transformed and differentiated epithelial prostate cell line

EPN
Category:  Cells  /  Cell lines
Last revision: Jan 29, 2015
Author(s):  A.KISSLINGER, D. TRAMONTANO
Contact
Name: Annamaria Kisslinger
Address: IEOS -CNR via Pansini 5, 80131 Naples Italy
Phone: +3908107464552
Email: a.kisslinger@ieos.cnr.it

 

Figure legend:

 

Microphotograph of EPN cells in standard growing condition. Magnification: x150
Origin: Human prostate tissue

Description

EPN cells in culture, as prostate epithelial cells in vivo, express cytokeratin 5 and 14 whereas they do not express desmin. The epithelial origin of EPN cells is strengthened by the observation that EPN cells express an active form of the cytoplasm tyrosine kinase Pyk2. In fact, Pyk2 is expressed in the normal human prostate epithelia but not in the stromal component of the prostate (Stanzione et al., 2001).
Finally, clonal production of the prostate-specific antigen (PSA) could be detected in EPN cells.

A nontransformed differentiated prostate epithelial cell in vitro, like the EPN cells, will enrich the scenario of prostate model systems in vitro and will prove useful in a wide range of studies in cellular genetics, gene regulation, pharmacology, and aging.

Culture conditions: Keratinocyte-serum free medium (SFM) ( Gibco-Brl Milan Italy)supplemented with bovine pituitary excrat (10mg/ml), epidermal growth factor EGF (10ng/ml),cholera toxin (10ng/ml), 5% foetal calf serum (FCS) (Gibco-Brl, Life technologies, Paisley Sctland UK).

 

Quality validation: Yes

Validation info

The model system has been published in  peer-reviewed journals

 

Citations
Kisslinger A, Cantile M, Sparaneo G, Vitale N, Fabbrocini G, Chieffi P, Cillo C, Mancini FP, Tramontano D. (2009) cAMP and Pyk2 interact to regulate prostate cell proliferation and function. Cancer Biol Ther.2009, 8, 236-242
Chieffi P, Cozzolino L, Kisslinger A, Libertini S, Staibano S, Mansueto G, De Rosa G, Villacci A, Vitale M, Linardopoulos S, Portella G, Tramontano D. Aurora B expression directly correlates with prostate cancer malignancy and influence prostate cell proliferation. Prostate 2006,66, 326-33
Chieffi P1, Kisslinger A, Sinisi AA, Abbondanza C, Tramontano D.17beta-estradiol-induced activation of ERK1/2 through endogenous androgen receptor-estradiol receptor alpha-Src complex in human prostate cells.Int J Oncol. 2003 Sep;23(3):797-801.
de Amicis F, Lanzino M, Kisslinger A, Calì G, Chieffi P, Andò S, Mancini FP, Tramontano D.Loss of proline-rich tyrosine kinase 2 function induces spreading and motility of epithelial prostate cells.J Cell Physiol. 2006 Oct;209(1):74-80.
Sinisi AA, Chieffi P, Pasquali D, Kisslinger A, Staibano S, Bellastella A and Tramontano D. EPN: a novel epithelial cell line derived from human prostate tissue. In Vitro Cell Dev Biol Anim. 2002 38, 165-72.

 

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