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Molecular Biology

SELEX methodology for cell-specific aptamers for targeted therapies

Selex protocol for selection of GL21.T and Gint4.T and CL4 aptamers 

Category:  Molecular Biology

RNA-protein interaction

RNA-protein interaction

RNA binding assay REMSA (RNA electrophoresis shift assay)  

Category:  Molecular Biology

Cytoplasmic Protein extraction

S100 cytoplasmic extract 

Category:  Molecular Biology

Preparation of radioactive probes for nucleic acids blots

It generates highly specific and hot probes. 

Category:  Molecular Biology

Magnetic labeling and isolation of biotinylated molecules

Magnetic labeling and isolation of biotinylated molecules

A fishing/competition approach used to validate the interaction between lncRNAs and microRNAs by using biotinylated Peptide Nucleic Acid (PNA) oligomers as probes.  

Category:  Molecular Biology

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