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Molecular Biology

Preparation of Digoxigenin labeled riboprobes

Preparation of riboprobes useful for in situ hybridization 

Category:  Molecular Biology

DNA extraction from whole blood

DNA extraction from whole blood using a salting out procedure 

Category:  Molecular Biology

Inverse-PCR (I-PCR) for the identification of the breakpoints of deletions

Inverse-PCR (I-PCR) for the identification of the breakpoints of deletions

The Inverse-PCR is a method that allows to amplify unknown regions of DNA, starting from the flanking known region on which are positioned the primers, in inverse direction compared to a normal PCR. It is necessary circularizing the DNA fragments, using ligase, then perform the I-PCR. 

Category:  Molecular Biology

Circularization of DNA fragments for the molecular characterization of new deletions

Circularization of DNA fragments for the molecular characterization of new deletions

During the molecular characterization of new deletions (to define the breakpoints) can occur that one extreme is known, but not the second one. In these cases it is very useful and suitable to perform the circulatization by ligase of DNA fragments containing the beakpoints of the deletion, followed by the inverse-PCR. Using these methods we were able to define the breakpoints of two new beta-Thalassemia deletions.  

Category:  Molecular Biology

RNA riboprobe synthesis

T7 RNA polymerase transcription 

Category:  Molecular Biology

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