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Home  |  Protocols  |  In situ hybridization and Immunochemistry  |  Whole mount in situ hybridizatyon on mouse embryos with DIG-and FLUO-labeled riboprobes  |  MAB 5X pH7.5 (400ml)

MAB 5X pH7.5 (400ml)


Reagents F-concentration Quantity I-concentration
Maleic acid
0.5M
23.2g
NaCl 0.75M
0.75M
17.4g

Wait that all the maleic acid is melt and later add NaCl. Reach the adequate pH adding NaOH

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