Hybridization Mix (100ml)
| Reagents | F-concentration | Quantity | I-concentration |
|---|---|---|---|
|
Formamide
|
50%
|
50ml
|
100%
|
|
SSC pH 4.5-5
|
5X
|
25ml
|
20X
|
|
Tween 20
|
0.1%
|
1ml
|
10%
|
|
Heparin
|
50 μg/ml
|
0.1 ml
|
50 mg/ml
|
|
Add at the moment only in the solution you have to use
|
|||
|
tRNA
|
50 μg/ml
|
10 mg/ml
|
|
|
DNA salmon sperm
|
50 μg/ml
|
10 mg/ml
|
Store hybridization mix (w/o DNAss and tRNA) at -20°C.
Add DNAss and tRNA just before using the hybridization solution.
