Hybridization solution
| Reagents | F-concentration | Quantity | I-concentration |
|---|---|---|---|
|
Tris HCl pH 7.5
|
10 mM
|
2 M
|
|
|
NaCl
|
600 mM
|
5 M
|
|
|
EDTA
|
1 mM
|
0.5 M
|
|
|
SDS
|
0.25 %
|
20%
|
|
|
Dextransulfate
|
10%
|
100%
|
|
|
Formamide
|
50%
|
100%
|
|
|
Denhardt's
|
1X
|
100 X
|
|
|
tRNA
|
200 µg/ml
|
10 mg/ml
|
|
|
DNA (salmon sperm)
|
100 µg/ml
|
10 mg/ml
|
|
|
H2O bd
|
up to final volume
|
