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Home  |  Molecular Tools  |  Oligos  |  alpha2 IVS-I -5 nt (Hph I)
Home  |  Molecular Tools  |  Oligos  |  alpha2 IVS-I -5 nt (Hph I)

Oligo - alpha2 IVS-I -5 nt (Hph I)

Oligo for allele-splecific amplification to genotype the human alpha2 IVS-I -5 nt (Hph I) globin gene mutation

Category:  DNA
Creation date: Oct 13, 2004
Last revision: Jan 26, 2016
Author(s):  Giuseppina Lacerra
Contact
Name: Giuseppina Lacerra
Address: Institute of Genetics and Biophysics A. Buzzati-Traverso - Via Pietro Castellino, 111, 80131 Naples, Italy
Phone: +39 081 6132602
Email: giuseppina.lacerra@igb.cnr.it

Sequence

GCGGAGGCCCTGGAGACG/C

 

Orientation: forward
Position: 33854-71 respect to the Gene Bank sequence NG_000006.1.
Length (nt): 19
Melting Temperature (°C): 62
% GC: 79
Degeneration: no
Intended Use: The oligonucleotide has been designed for an amplification refractory mutation system (ARMS) assay. It generates a mutation-specific amplicon of 789 bp if it is used in association with the oligonucleotide alpha2 + 904.

 

Quality validation: Yes

Validation info

The ARMS oligo alpha2 IVS-I -5nt (Hph I) has been published and used to obtain data published in the peer-reviewed journals reported below.

 

Citations
Lacerra G, Musollino G, Di Noce F, Prezioso R, Carestia C. Genotyping for known Mediterranean alpha-thalassemia point mutations using a multiplex amplification refractory mutation system. Haematologica. 2007 Feb;92(2):254-5. PubMed PMID: 17296579.
Lacerra G, Scarano C, Musollino G, Flagiello A, Pucci P, Carestia C. Hb Foggia or alpha 117(GH5)Phe -> Ser: a new alpha 2 globin allele affecting the alpha Hb-AHSP interaction. Haematologica. 2008;93:141-2.
Lacerra G, Scarano C, Musollino G, Testa R, Prezioso R, Caruso DG, Lagona LF, Medulla E, Friscia MG, Gaudiano C, Carestia C. HbA2-Partinico or delta(A2)Pro-->Thr, a new genetic variation in the delta-globin gene in cis to the beta(+) thal IVS-I-110 G>A, and the heterogeneity of delta-globin alleles in double heterozygotes for beta- and delta-globin gene defects. Ann Hematol. 2010;89:127-34.
Lacerra G, Scarano C, Lagona LF, Testa R, Caruso DG, Medulla E, Friscia MG, Mastrullo L, Caldora M, Prezioso R, Gaudiano C, Magnano C, Romeo MA, Musollino G, Di Noce F, Carestia C. Genotype-phenotype relationship of the δ-thalassemia and Hb A(2) variants: observation of 52 genotypes. Hemoglobin. 2010;34:407-23.
Musollino G, Mastrolonardo G, Prezioso R, Pagano L, Primignani P, Carestia C, Lacerra G. Molecular mechanisms of a novel β-thalassaemia mutation due to the duplication of tetranucleotide 'AGCT' at the junction IVS-II/exon 3. Ann Hematol. 2012;91:1695-701.

 

Funded by: Ministero Istruzione, Università e Ricerca (MIUR), Legge 488/92, Cluster C02, Project 2.

 

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