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Home  |  Molecular Tools  |  Oligos  |  1 South-Italy beta0-thal I-PCR

Oligo - 1 South-Italy beta0-thal I-PCR

Oligo for Inverse PCR amplification assay to define the breakpoint of the deletion South-Italy beta0-thal. Because the oligos are in inverse direction they cannot generate amplicomers if used on a linear fragment.

CategoryDNA
Creation dateMar 10, 2009
Last revisionFeb 18, 2016
Author(s)Giuseppina Lacerra
Contact
NameGiuseppina Lacerra
AddressInstitute of Genetics and Biophysics A. Buzzati-Traverso - Via Pietro Castellino, 111, 80131 Naples, Italy
Phone+39 081 6132602
Emailgiuseppina.lacerra@igb.cnr.it

Sequence

AAGTGAAGCATCTCCTGGAC

 

Orientationforward
Position5233967-5233986 respect to the Gene Bank sequence NC_000011.10
Length (nt)20
Melting Temperature (°C)53.0
% GC50
Degenerationno
Intended UseIt has been used on a circularized fragment (containing the breakpoint of the new deletion) in association with the oligo “2 South-Italy beta0-thal I-PCR” generating a mutation-specific amplicon of 1596 bp.

 

Quality validation: Yes

Validation info

The oligo "1 South-Italy beta0-thal I-PCR" has been published and used to obtain data published in the peer-reviewed journals reported below.

 

Citations
De Angioletti M, Sabato V, Musollino G, Prezioso R, Carestia C, Lacerra G. South-Italy β°-thalassemia: a novel deletion not removing the γ-globin silencing element and with 3' breakpoint in a hsRTVL-H element, associated with β°-thalassemia and high levels of HbF. Haematologica. 2013 Aug;98(8):e98-e100. doi: 10.3324/haematol.2013.089722. Epub 2013 Jun 28. PubMed PMID: 23812938; PubMed Central PMCID: PMC3729893.

 

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