Oligo - alpha2 N-Hb Caserta
Oligo for allele-splecific amplification to genotype the human alpha2 N-Hb Caserta globin gene mutation
Category:
DNA
Creation date: Nov 28, 2005
Last revision: Jan 26, 2016
Author(s):
Giuseppina Lacerra
Contact
Name: Giuseppina Lacerra
Address: Institute of Genetics and Biophysics A. Buzzati-Traverso - Via Pietro Castellino, 111, 80131 Naples, Italy
Phone: +39 081 6132602
Sequence
GCACGCTGGCGAGTATGCTG
Orientation: forward
Position: 33835-54 respect to the Gene Bank sequence NG_000006.1.
Length (nt): 20
Melting Temperature (°C): 62
% GC: 65
Degeneration: no
Intended Use: The oligonucleotide has been designed for an amplification refractory mutation system (ARMS) assay. Used in association with the oligonucleotide alpha2 + 904 it generates a normal-specific amplicon of 808 bp.
Quality validation:
Yes
Validation info
The ARMS oligo alpha2 N-Hb Caserta has been published and used to obtain data published in the peer-reviewed journals reported below.
Funded by: Ministero Istruzione, Università e Ricerca (MIUR), Legge 488/92, Cluster C02, Project 2.
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