Oligo - alpha2 cod 142 TAA>AAA (Hb Icaria)
Oligo for allele-splecific amplification to genotype the human alpha2 cod 142 TAA>AAA (Hb Icaria) globin gene mutation
Category:
DNA
Creation date: Oct 13, 2004
Last revision: Jan 26, 2016
Author(s):
Giuseppina Lacerra
Contact
Name: Giuseppina Lacerra
Address: Institute of Genetics and Biophysics A. Buzzati-Traverso - Via Pietro Castellino, 111, 80131 Naples, Italy
Phone: +39 081 6132602
Sequence
CGTGCTGACCTCCAAATACGGTA
Orientation: forward
Position: 34439-61 respect to the Gene Bank sequence NG_000006.1.
Length (nt): 23
Melting Temperature (°C): 62
% GC: 52
Degeneration: no
Intended Use: The oligonucleotide has been designed for an amplification refractory mutation system (ARMS) assay. It generates a mutation-specific amplicon of 204 bp if used in association with the oligonucleotide alpha2 + 904.
Quality validation:
Yes
Validation info
The ARMS oligo alpha2 cod 142 TAA>AAA has been published and used to obtain data published in the peer-reviewed journals reported below.
Funded by: Ministero Istruzione, Università e Ricerca (MIUR), Legge 488/92, Cluster C02, Project 2.
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